CRL-9078 PA 317 小鼠成纖維細胞
ATCC® Number: CRL-9078?
Designations: PA317
Depositors: Fred Hutchinson Cancer Res. Cntr.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
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Applications: transfection host (Roche FuGENE® Transfection Reagents)
Packaging line for retroviruses.
Age: embryo
Comments: Derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA, pPAM3, carried in pBR322 and herpes simplex virus thymidine kinase (TK) gene carried in pBR322.
The PA317 cell line was derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA (pPAM3) carried in pBR322 and the herpes simplex virus thymidine kinase (TK) gene carried in pBR322.
Introduction of retroviral vectors into these cells, by infection or by transfection, results in production of retrovirus virions with an amphotropic host range that are capable of infecting cells of many mammalian species.
Virions produced by this line have been used successfully to transfer genes into humans.
The percentage of PA317 cells that are capable of packaging retroviral vectors decreases slowly with continued passaging of the cell line, presumably due to the loss of the transfected DNA used to create the line.
