產品[
CRL-2785 TC-1 小鼠肺上皮細胞
]資料
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產品名稱:
CRL-2785 TC-1 小鼠肺上皮細胞
產品型號:
TC-1 小鼠肺上皮細胞
產品廠商:
美國標準生物品收藏中心（ATCC）
產品文檔:
無相關文檔
簡單介紹
CRL-2785 TC-1 小鼠肺上皮細胞 ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件!
CRL-2785 TC-1 小鼠肺上皮細胞
的詳細介紹
CRL-2785 TC-1 小鼠肺上皮細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件!
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CRL-2785?
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TC-1 [JHU-1]
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TC Wu
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Biosafety Level:
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2 [Cells contain HPV-16 and c-Ha-ras viral DNA sequences ]
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Mus musculus
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epithelial
CRL-2785 TC-1 小鼠肺上皮細胞
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Organ: lung
Strain: C57BL/6
Disease: tumor
Cell Type: human papillomavirus 16 (HPV-16) E6/E7 and c-Ha-Ras cotransformed
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Part of the Johns Hopkins Special Collection
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The TC-1 lung metastasis model can be used to test the efficacy of various E6/E7-specific vaccines and immunotherapeutic strategies [PubMed: 9724092].
The tumor cell line, TC-1 [tissue culture number one], was derived from primary lung epithelial cells of C57BL/6 mice.
The cells are positive for the expression of HPV-16 E7 [PubMed: 8548765].
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Yes CRL-2785 TC-1 小鼠肺上皮細胞
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ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Subculture before confluence.
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Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
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Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
Cultures can be established between 1 X 10(5) and 2 X 10(5) viable cells/cm2.Maintain cultures at a cell concentration between 2 X 10(5) and 4 X 10(5) viable cells/cm2. Do not exceed 6 X 10(5) cells/cm2..
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Incubate cultures at 37°C.
Interval: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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24 hours
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
source culture:ATCC JHU-1
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57430: Lin KY, et al. Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen. Cancer Res. 56: 21-26, 1996. PubMed: 8548765
88870: Chen CH, et al. Antigen-specific immunotherapy for human papillomavirus 16 E7-expressing tumors grown in the liver. J. Hepatol. 33: 91-98, 2000. PubMed: 10905591
88871: Ji H, et al. Antigen-specific immunotherapy for murine lung metastatic tumors expressing human papillomavirus type 16 E7 oncoprotein. Int. J. Cancer 78: 41-45, 1998. PubMed: 9724092
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CRL-2785 TC-1 小鼠肺上皮細胞
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